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Thermo Scientific™ Pierce™ Thiophilic Adsorption Kit PROMO
Description
Pierce Thiophilic Adsorbent is 6% beaded agarose that has been modified to contain simple sulfone-thioether groups. The result is an affinity resin that has a high binding capacity and a broad specificity for many species of immunoglobulins when incubated in 0.5M potassium sulfate. In effect, thiophilic adsorption is a resin-based variation of ammonium sulfate precipitation for quick immunoglobulin purification from crude serum samples. Unlike antibody fractionation by ammonium sulfate precipitation, purification with thiophilic adsorbent yields concentrated, essentially salt-free, highly purified immunoglobulins at near-neutral pH. Thus, this simple one-step method eliminates the need for additional treatment of the sample for storage or for subsequent conjugation reactions. The gentle binding and elution conditions ensure a high protein recovery with excellent preservation of antibody activity. The resin is available as both a standalone product and as a purification kit containing pre-packed columns and ready-to-use buffers.
Highlights:
High capacity – gently bind and purify approx. 20mg polyclonal antibody per milliliter of adsorbent resin
Broad antibody specificity – effective for most immunoglobulin classes and isotypes derived from many different animal species, including chicken IgY
Simple and fast – quickly fractionate and purify the immunoglobulin component of serum or ascites samples without subsequent dialysis or desalting steps
Gentle – binding condition is nondenaturing and the elution buffer yields concentrated, salt-free immunoglobulin at near neutral pH; provides excellent recovery of functional antibody
Includes:
Kit contains four Thiophilic Adsorbent Columns (3 mL each), ready-to-use binding and elution buffers, column regenerant and storage buffer, and column accessories
Recommended for:
Mild isolation of immunoglobulins from human serum; Rapid purification of mouse monoclonals from the culture media or ascites fluid; Selective removal of immunoglobulins from fetal calf serum - useful for cell culture in monoclonal antibody production; Rapid, straightforward procedure yielding essentially pure immunoglobulins from crude rabbit serum; Purification of IgY from chicken; Large-scale purification for biotechnology applications
Specifications
Specifications
| Product Type | Adsorption Kit |
| Product Line | Pierce™ |
| Column Type | Agarose Resin, Affinity |
| Format | Gravity Flow Column |
| Stationary Phase | Sulfone Thioether |
| Purification Target | IgG Antibody |
| Content And Storage | Sufficient For: Purifying up to 60 mg immunoglobulin per column, Thiophilic Adsorbent Columns, 3 mL, 4 Columns, Binding Buffer, 1 L, Elution Buffer, 1 L, Column Storage Buffer (2X), 100 mL, Guanidine-HCl Crystals, 230 g, Column Accessories, Store at 4°C. |
| Quantity | 4 Columns |
For Research Use Only. Not for use in diagnostic procedures.
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