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Thermo Scientific™ Pierce™ TMT11plex Yeast Digest Standard

Thermo Scientific Pierce TMT11plex Yeast Digest Standard is a lyophilized yeast peptide mixture of four congenic yeast strains labeled with TMT11plex reagents to monitor LC-MS/MS system performance for multiplexed quantitation.

$486.50 - $1721.00

Specifications

For Use With (Equipment) Mass Spectrometer
Product Type Digest Standard
Product Line Pierce™
Shipping Condition Wet Ice
Starting Material Peptides
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
PIA40938
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Thermo Scientific™
A40938
20 μg
Each for $486.50
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PIA40939
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Thermo Scientific™
A40939
5 x 20 μg
Each for $1,721.00
Edge
Add to Cart
 
Description

Description

The Pierce TMT11plex Yeast Digest Standard consists of four congenic strains of Saccharomyces cerevisiae (a parental line, BY4741, and three lines lacking the non-essential proteins Met6, His4, or Ura2) labeled in triplicate (knock-out strains) or duplicate (parental strain) with TMT11-plex label reagents. The knock-out strains are used in combination with the parental strain to enable comparison of peptide and protein abundance for quantitation. Pierce TMT11plex Yeast Digest Standard:
Convenient—ready-to-use lyophilized powder pre-labeled with TMT11plex reagents
Quality—yeast strains verified for genotype, equimolar mixing of the TMT-labeled strains
Quantitative—measure the quantitative precision of TMT reporter ions for 11 channels
Diagnostic—excellent QC assay tool for protein quantitation using TMT on LC-MS/MS instruments

Quantitative proteomic strategies using TMT reagents enable multiplexing and precise measurement of protein abundance. Successful execution of TMT quantitation includes optimized chromatography, mass spectrometry (MS), and data analysis. A critical parameter for success in this workflow is the ability to consistently detect TMT fragment ions across 11 channels for multiplexing. Our TMT11plex Yeast Digest standard has been specifically designed to assess LC-MS/MS system performance for TMT quantitation, MS method optimization and system validation. For method development, TMT11plex Yeast Digest Standard can be used to measure the accuracy, precision, and dynamic range of different mass spectrometry approaches. These standards can help optimize offline fractionation, chromatography, mass spectrometry acquisition, and data analysis settings. Multifunctional applications using this standard reduces inter-experiment variability to provides reproducible TMT quantitation.

References:
1. Paulo JA, O'Connell JD, Gygi SP. A Triple Knockout (TKO) Proteomics Standard for Diagnosing Ion Interference in Isobaric Labeling Experiments. J Am Soc Mass Spectrom. 2016 10:1620-5.
2. Yang F, Shen Y, Camp DG 2nd, Smith RD. High-pH reversed-phase chromatography with fraction concatenation for 2D proteomic analysis. Expert Rev Proteomics. 2012 9(2):129-34.
3. Dayon L, Pasquarello C, Hoogland C, Sanchez JC, Scherl A. Combining low- and high-energy tandem mass spectra for optimized peptide quantification with isobaric tags. J Proteomics. 2010 73(4):769-77.
4. Diedrich JK, Pinto AF, Yates JR 3rd. Energy dependence of HCD on peptide fragmentation: stepped collisional energy finds the sweet spot. J Am Soc Mass Spectrom. 2013 24(11):1690-9.
5. Chiva C, Sabidó E. HCD-only fragmentation method balances peptide identification and quantitation of TMT-labeled samples in hybrid linear ion trap/orbitrap mass spectrometers. J Proteomics. 2014 96:263-70.

Related products:
Pierce HeLa Protein Digest Standard (Cat. No. 88329)
Pierce Peptide Retention Time Calibration Mixture (Cat. No. 88321)
TMT10plex isobaric label reagent set plus TMT11-131C label reagent (Cat. No. A34808)

Specifications

Specifications

Mass Spectrometer
Pierce™
Peptides
Mass Spectrometry
Lyophilized Powder
Digest Standard
Wet Ice
MS Standard
MS System Performance
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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