Thermo Scientific™ Yeast beta-Galactosidase Assay Kit

Description

Extract protein from yeast two-hybrid reporter systems and measure beta-galactosidase activity using compatible Y-PER Reagent and b-Gal Assay Reagent.

Thermo Scientific™ Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.

The Yeast β-Galactosidase Assay Kit allows for qualitative or quantitative determination of β-galactosidase activity in solution directly from colonies growing on solid medium. A portion of the colony is suspended in a mixture of Thermo Scientific Y-PER™ Yeast Protein Extraction Reagent and the β-Galactosidase Assay Buffer. After a brief incubation period, the solution turns yellow from the hydrolysis of o-nitrophenyl-β-D-galactopyranoside (ONPG) to o-nitrophenol (ONP) and galactose in a mildly alkaline solution. The assay becomes quantitative if calibrated to cell number by measuring the optical density at 660nm.

Highlights:

• Efficient lysis of yeast cells and a colorimetric detection system
• Quantitative or qualitative assay for yeast colonies grown on solid media or in suspension
• Colorimetric assay easily measured in a plate reader or spectrophotometer (420nm)
• Adaptable to 96-well microplates or tube assay formats
• Perform homogeneous screening assays or measure activity secondarily after cell lysis
• Also compatible with prepared lysates from other species such as bacteria

Includes:

Thermo Scientific Y-PER Yeast Protein Extraction Reagent (Part No. 78990), 2X Assay Buffer and Stop Solution

Specifications

• Content And Storage: Sufficient For: 250 colonies or 250 culture samples (100 μL each)
  Y-PER Yeast Protein Extraction Reagent, 25 mL (store at room temperature)
  2X beta-Galactosidase Assay Buffer, 25 mL (store at -20°C)
  1M Sodium Carbonate Stop Solution, 25 mL (store at room temperature)
• Detection Method: Reporter Enzyme
• For Use With (Application): Absorbance
• For Use With (Equipment): Microplate Reader
• Product Type: Beta-Galactosidase Assay Kit
• Dye Type: Enzyme Labeled
• Format: 96-well Plate
• Product Line: Pierce
• Quantity: 75 mL
• Substrate: ONPG
• Substrate Type: Beta-Gal Substrate
• Sufficient For: 250 colonies or 250 culture samples (100 μL each)
• Target: Beta-Galactosidase

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.