Invitrogen™ PIF1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5103641

Catalog No. PIPA5103641

Description

Antibody detects endogenous levels of total PIF1.

PIF1 is a single-stranded (ss) DNA-dependent ATPase as well as a DNA helicase that unwinds DNA in a 5' to 3' direction with respect to the DNA strand on which it binds first. This protein is critical to DNA replication and telomere length maintenance in Saccharomyces cerevisiae. The PIF1 gene is highly conserved from yeast to humans. Mutations in the gene that encodes for PIF1 cause all telomeres to lengthen, suggesting that the PIF1 functions as a catalytic inhibitor of both new telomere formation and telomere elongation. Human PIF1 preferentially binds telomeric DNA where it inhibits telomerase activity. PIF1 specifically counteracts the RecQ homolog Sgs1 helicase activity, and degradation of PIF1 is mediated by the ubiquitin-26S proteasome pathway.

Specifications

• Antigen: PIF1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PIF1
• Gene Alias: 4631410M14; AI449441; ATP-dependent DNA helicase PIF1; ATP-dependent DNA helicase PIF1 {ECO:0000255; C15orf20; DNA helicase-like protein; DNA repair and recombination helicase PIF1; DNA repair and recombination helicase PIF1 {ECO:0000255; HAMAP-Rule:MF_03176}; petite integration frequency 1; PIF; Pif1; PIF1 5'-to-3' DNA helicase; PIF1 5'-to-3' DNA helicase homolog; PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae); PIF1 homolog; Pif1/Rrm3 DNA helicase-like protein; Pif1/Rrm3 DNA-helicase-like protein
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 208084, 367645, 80119
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: Q1HG60, Q80SX8, Q9H611
• Gene Symbols: PIF1
• Immunogen: A synthesized peptide derived from human PIF1(Accession Q9H611), corresponding to amino acid residues V322-V372.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG