PIPES, 1.0 M Buffer Solution, pH 6.8, PROMO, Thermo Scientific Chemicals

Description

Applications

PIPES is frequently used as a buffering agent in biochemistry. PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues and has a negligible capacity to bind divalent ions. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer is done.

Solubility

Soluble in 1 M NaOH (0.5 M at 20°C), water (approx.1 g/L at 100°C), and 0.5 N NaOH (0.1 M).

Notes

Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away from strong oxidizing agents.

Specifications

Chemical Identifiers

• CAS: 5625-37-6
• Molecular Formula: C8H18N2O6S2
• Molecular Weight (g/mol): 302.36 g/mol
• MDL Number: MFCD00006159
• InChI Key: IHPYMWDTONKSCO-UHFFFAOYSA-N
• PubChem CID: 79723
• ChEBI: CHEBI:44933
• IUPAC Name: 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
• SMILES: C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

• Color: Colorless
• Concentration: 1.0 M
• pH: 6.8
• Physical Form: Liquid
• Quantity: 500 mL
• Solubility Information: Soluble in 1M NaOH, water, and 0.5 N NaOH.
• Chemical Name or Material: PIPES buffer solution

Safety and Handling

TSCA	Yes
Recommended Storage	Keep cold

RUO - Research Use Only