MilliporeSigma™ PIT-1 (POU1F1), Mouse, Unlabeled, Clone: 8B6.1,

Catalog No.	Quantity	Conjugate
MABN1602MI	100 μL	Unconjugated

Catalog No. MABN1602MI Supplier MilliporeSigma™ Supplier No. MABN1602

Description

Mouse Monoclonal Antibody

Pituitary-specific positive transcription factor 1 (UniProt P28069; also known as GHF-1, Growth hormone factor 1, PIT-1) is encoded by the POU1F1 (also known as CPHD1, GHF1, PIT1) gene (Gene ID 5449) in human. PIT-1 is a transcription factor that serves as a master regulator of lineage commitment and expansion during the anterior pituitary development process that drives regional expansion and functional differentiation of five distinct hormone-producing cell lineages, including somatotropes, lactotropes, thyrotropes, corticotropes, and gonadotropes. Loss of Pit-1 expression results in a combined pituitary hormone deficiency (CPHD) syndrome reflecting the absence of lactotropes, somatotropes, and a subset of thyrotropes. Although originally characterized as pituitary-specific, PIT-1 is also expressed in human breast. PIT-1 upregulation in breast tumors increases cell proliferation and the expression of two breast cancer-related hormones, growth hormone (GH) and prolactin (PRL), leading to MMPs upregulation and breast cancer metastasis. Human POU1F1 gene encodes two alternatively spliced isoforms, where Pit-1A (GHF-2) a.a. 47-73 is truncated to a single Thr47 residue in Pit-1B (GHF-1). Both PIT-1 isoforms contain a PIT-1 POU-specific domain (a.a. 150-224 of Pit-1A or a.a. 124-198 of Pit-1B) and a DNA-binding Homeobox (a.a. 240-299 of Pit-1A or a.a. 214-273 of Pit-1B).

Specifications

• Antigen: PIT-1 (POU1F1)
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 8B6.1
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG2a in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: POU1F1, CPHD1, GHF1, PIT1
• Gene Accession No.: P28069
• Host Species: Mouse
• Immunogen: GST-tagged recombinant human PIT-1 internal fragment.
• Purification Method: Protein G Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_000297
• Test Specificity: Clone 8B6.1 epitope lies within an internal region present in both human PIT-1 spliced isoform A (Pit-1A, GHF-2) and isoform B (Pit-1B, GHF-1).
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified
• Isotype: IgG2a κ

For Research Use Only. Not for use in diagnostic procedures.