Invitrogen™ PIWIL1 Recombinant Rabbit Monoclonal Antibody (10H15L2)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ 701177

Catalog No. PI701177

Description

This antibody is predicted to react with mouse based on sequence homology. Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Hiwi is a member of the Piwi /Argonaute protein family. Hiwi is specifically expressed in germline cells such as spermatocytes and round spermatids during spermatogenesis. Hiwi is also expressed in CD34+ hematopoietic progenitors, but not in CD34- hematopoietic cells, suggesting a role for this gene in the maintenance of hematopoietic stem cells.

Specifications

• Antigen: PIWIL1
• Classification: Recombinant Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: Q96J94, Q9JMB7
• Gene Symbols: PIWIL1
• Immunogen: Recombinant protein corresponding to amino acids 90-213 of human Hiwi.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot, Immunocytochemistry
• Clone: 10H15L2
• Conjugate: Unconjugated
• Gene: PIWIL1
• Gene Alias: CT80; CT80.1; HILI; HIWI; mili; Miwi; Miwi like; Miwi likw; PIWI; piwi homolog; piwi like homolog 1; piwi like RNA-mediated gene silencing 1; PIWIL1; PIWIL1L; piwil2-like protein; piwi-like 1; piwi-like homolog 1; piwi-like protein 1; piwi-like protein 2; piwi-like RNA-mediated gene silencing 1
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 363912, 57749, 9271
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov