Invitrogen™ PKA alpha Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA521842

Catalog No. PIPA521842

Description

Recommended positive controls: HeLaS3, NIH-3T3, C2C12. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (94%), Dog (99%), Rabbit (97%), Sheep (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.

Specifications

• Antigen: PKA alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PRKACA
• Gene Alias: alternatively spliced; C(s); Calpha; C-alpha-2; C-alpha-S; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; cAMP-dependent protein kinase catalytic subunit C alpha; cAMP-dependent protein kinase catalytic subunit Calpha1; Cs-PKA; MGC102831; MGC48865; PKA; PKA C-alpha; PKA catalytic subunit alpha; pkac; Pkaca; PKCA1; PKCD; PPNAD4; PRKACA; protein kinase; protein kinase A; protein kinase A alpha; protein kinase A catalytic subunit; protein kinase cAMP-activated catalytic subunit alpha; protein kinase, cAMP dependent, catalytic, alpha; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha; sperm cAMP-dependent protein kinase catalytic subunit; sperm cAMP-dependent protein kinase catalytic subunit Cs
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18747, 5566
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P05132, P17612
• Gene Symbols: PRKACA
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 351 of PKA C alpha (Uniprot ID#P17612).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG