PKC-alpha Rabbit anti-Human, Mouse, Polyclonal, Bethyl Laboratories

Rabbit Polyclonal Antibody

Supplier:  Bethyl Laboratories, Inc A302446A

Catalog No. 50-156-2606

Description

The recommended shelf life for this product is 1 year from date of receipt. Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Based on 100% sequence identity, this antibody is predicted to react with Rat and Bovine

Members of the Protein kinase C (PKC) family of serine/threonine protein kinases are grouped by their activation mechanism. Classical or conventional PKCs (PKC alpha-, betaI- , betaII- and gamma-) are activated by phosphatidylserine in a calcium dependent manner and can bind diacylglycerol. The Ca2+ insensitive novel PKCs (PKCs epsilon-, delta-, theta- and eta) are also activated by diacylglycerol and phosphatidylserine. The atypical PKCs (PKCs iota- and zeta-) are insensitive to Ca2+, DAG and phorbolesters. All PKCs isoforms consist of a highly conserved catalytic domain connected to a regulatory domain via a hinge region. PKCs are involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascades involving MAPK1/3 (ERK1/2) and RAP1GAP.

Specifications

• Antigen: PKC-alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PRKCA
• Gene Alias: AAG6, aging-associated gene 6, PKCA, PKC-A, PKC-alpha, PRKACA, protein kinase C, alpha
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18750, 5578
• Content And Storage: 4° C
• Form: Liquid

• Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Concentration: 0.2 mg/ml
• Formulation: TBS with 0.1% BSA and 0.09% sodium azide
• Gene Accession No.: P17252, P20444
• Gene Symbols: PRKCA
• Immunogen: Between 622 and 672
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG