Invitrogen™ PKC lambda/iota Monoclonal Antibody (F.699.5)

Rabbit Monoclonal Antibody

Supplier:  Invitrogen™ MA514874

Catalog No. PIMA514874

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with other PKC isoforms.

The PKC family of serine/threonine kinases, including PRKCI (PKC iota), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.

Specifications

• Antigen: PKC lambda/iota
• Classification: Monoclonal
• Concentration: 20 μg/mL
• Formulation: 0.01M HEPES with 100μg/mL BSA, 50% glycerol, 0.15M NaCl and <0.02% sodium azide; pH 7.5
• Gene Accession No.: F1M7Y5, P41743, Q62074
• Gene Symbols: PRKCI
• Immunogen: Synthetic peptide corresponding to human PKC lambda/iota.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot, Immunocytochemistry
• Clone: F.699.5
• Conjugate: Unconjugated
• Gene: PRKCI
• Gene Alias: 2310021H13Rik; AI427505; aPKClambda; aPKC-lambda/iota; atypical protein kinase C-lambda/iota; DXS1179E; hypothetical protein; mKIAA4165; nPKC-iota; Pkci; Pkcl; PKClambda; PRKCI; Prkcl; PRKC-lambda/iota; protein kinase C iota; protein kinase C iota type; protein kinase C, iota; protein kinase C, lambda
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18759, 5584, 84006
• Content And Storage: -20°C
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov