Invitrogen™ Placental Alkaline Phosphatase Monoclonal Antibody (8B6), Biotin, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-2620	Invitrogen™ 13987082	100 μg

Description

Description: The monoclonal antibody 8B6 recognizes human Placental Alkaline Phosphatase (PLAP), a 130 (or 130 with 65 kDa) enzyme expressed by placental syncytiotrophoblastoid cells. This enzyme has slightly different enzyme kinetics from other phosphatases. PLAP can be found anchored to the plasma membrane in addition to the cytoplasm. Expression is found in several germ line cancers; ovarian and testicular. Expression is also present in human embryonic stem cells and bone marrow-derived mesenchymal stromal cells. This 8B6 antibody reacts with both the Regan and Nagao isozymes. Applications Reported: This 8B6 antibody has been reported for use in immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded (IHC-P) tissue, and ELISA. Applications Tested: This 8B6 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) human placenta with trypsin digestion. This antibody can be used at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

Placental Alkaline Phosphatase plays an important role in the regulation of specific inflammatory disease processes. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney for this form of alkaline phosphatase have been well characterized. Placental Alkaline Phosphatase reacts with a membrane-bound isoenzyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. Placental Alkaline Phosphatase is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA). A proposed function of Placental Alkaline Phosphatase is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. Placental Alkaline Phosphatase has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of hypophosphatasia can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms.

Specifications

• Antigen: Placental Alkaline Phosphatase
• Concentration: 0.5 mg/mL
• Applications: ELISA, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Conjugate: Biotin
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P05187
• Gene ID (Entrez): 250
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: 8B6
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2a κ
• Target Species: Human
• Gene: ALPP
• Gene Alias: Alkaline phosphatase placental type; Alkaline phosphatase Regan isozyme; alkaline phosphatase, placental; alkaline phosphatase, placental type; alkaline phosphomonoesterase; ALP; Alp1; ALPP; AP-TNAP; DOA1; FLJ11281; FLJ40094; FLJ61142; FLJ93059; Germ-cell alkaline phosphatase; glycerophosphatase; HOPS; MGC161443; MGC167935; nagao Isozyme; P PLAP; PALP; PLA2P; PLAA; placental alkaline phosphatase 1; placental heat-stable alkaline phosphatase; PLAP; PLAP1; PLAP-1; rCG23846-like; Regan isozyme; TNAP; TNSALP
• Gene Symbols: ALPP
• Product Type: Antibody