Invitrogen™ Platinum™ Taq DNA Polymerase High Fidelity
Ideal for amplification of DNA fragments when high yields and robust amplification are required
Manufacturer: Invitrogen™ 11304102
High fidelity is provided by a mixture of PlatinumTaq DNA Polymerase and the proofreading (3'-5' exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum automatic “hot-start” technology.
- Fidelity: greater than six times higher fidelity than Taq DNA polymerase
- Amplicon size: amplification of fragments up to 15kb
- Convenience: room temperature reaction assembly
- Applications: amplification of DNA from complex genomic, viral, and plasmid templates and RT-PCR
One unit of PlatinumTaq DNA Polymerase, High Fidelity, is the amount of enzyme required to incorporate 10nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C.
High Fidelity PCR, Hot Start PCR, Long Fragment PCR, PCR, PCR and Real-Time PCR, Sanger Sequencing, Sanger Sequencing Technology and Accessories, Sequencing
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Shipping Condition: Dry ice
|• 1 x 1000 µL Platinum Taq DNA Polymerase High Fidelity (5 U/µL)
• 1 x 50 mL 10X High Fidelity Buffer [600 mM Tris-SO4 (pH 8.9), 180 mM (NH4)2SO4]
• 1 x 25 mL 50 mM MgSO4
Store at -10°C to -30°C.
|3' - 5', 5' - 3'|
|Built-In Hot Start|
|Platinum™ Taq DNA Polymerase High Fidelity|
|20 kb or less|
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For Research Use Only. Not for use in diagnostic procedures.