Invitrogen™ PLEKHM3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA557127

Catalog No. PIPA557127

Description

Immunogen sequence: MIWDHCKSRL LETKAQNVFP AKEQFMVQRG TTPDNLSWME QKEASTFNFF NICQRRRDRP RSVNDLLDET STFKPGHARS RSDITQVD Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 94%.

PLEKHM3, also known as DAPR, is a member of the M family of Pleckstrin homology domain-containing proteins. PLEKHM3 was initially identified through chromatin immunoprecipitation and CpG microarray analysis examining proteins regulated by myocyte-enhancing factor 2. In C2C12 myoblast cells, PLEKHM3 binds to the PI3K signaling member protein kinase B in the cytosol prior to differentiation into myotubes. Following the initiation of differentiation, PLEKHM3 was also found in membrane fractions. Knockdown of PLEKHM3 expression by RNAi resulted in the inhibition of myotube formation, suggesting that PLEKHM3 is a key component required by myoblasts for orchestrating their differentiation during myogenesis.

Specifications

• Antigen: PLEKHM3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PLEKHM3
• Gene Alias: 9430067K14Rik; A230102O09Rik; AI449570; Dapr; differentiation associated protein; Differentiation-associated protein; PH domain-containing family M member 3; pleckstrin homology domain containing M3; pleckstrin homology domain containing, family M, member 1-like; pleckstrin homology domain containing, family M, member 3; pleckstrin homology domain-containing family M member 3; PLEKHM1L; Plekhm3; RGD1307151
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 241075, 316455, 389072
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.05 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q6ZWE6, Q8BM47
• Gene Symbols: PLEKHM3
• Immunogen: Recombinant protein corresponding to Human PLEKHM3. Recombinant protein control fragment (Product #RP-96208).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG