Invitrogen™ PLEKHM3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA520851

Catalog No. PIPA520851

Description

A suggested positive control is mouse skeletal muscle tissue lysate. PA5-20851 can be used with blocking peptide PEP-0965.

PLEKHM3, also known as DAPR, is a member of the M family of Pleckstrin homology domain-containing proteins. PLEKHM3 was initially identified through chromatin immunoprecipitation and CpG microarray analysis examining proteins regulated by myocyte-enhancing factor 2. In C2C12 myoblast cells, PLEKHM3 binds to the PI3K signaling member protein kinase B in the cytosol prior to differentiation into myotubes. Following the initiation of differentiation, PLEKHM3 was also found in membrane fractions. Knockdown of PLEKHM3 expression by RNAi resulted in the inhibition of myotube formation, suggesting that PLEKHM3 is a key component required by myoblasts for orchestrating their differentiation during myogenesis.

Specifications

• Antigen: PLEKHM3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PLEKHM3
• Gene Alias: 9430067K14Rik; A230102O09Rik; AI449570; Dapr; differentiation associated protein; Differentiation-associated protein; PH domain-containing family M member 3; pleckstrin homology domain containing M3; pleckstrin homology domain containing, family M, member 1-like; pleckstrin homology domain containing, family M, member 3; pleckstrin homology domain-containing family M member 3; PLEKHM1L; Plekhm3; RGD1307151
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 241075, 316455, 389072
• Content And Storage: Maintain refrigerated at 2°C to 8°C for up to 3 months. For long term storage store at -20°C
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: Q6ZWE6, Q8BM47
• Gene Symbols: PLEKHM3
• Immunogen: A 13 amino acid peptide from near the carboxy terminus of human PLEKHM3.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG