| Antigen |
PLK1 (p Thr210) |
| Applications |
Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin) |
| Classification |
Polyclonal |
| Concentration |
1 mg/ml |
| Conjugate |
Unconjugated |
| Dilution |
Western Blot 1:200-1:2000, ELISA 1:3000-1:12000, Immunohistochemistry 1:200-1:1000, Immunohistochemistry-Paraffin 1:200 |
| Formulation |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide |
| Gene Accession No. |
P53350 |
| Gene Alias |
cell cycle regulated protein kinase, EC 2.7.11, EC 2.7.11.21, PLK, PLK-1, polo (Drosophia)-like kinase, polo like kinase, polo-like kinase 1polo-like kinase (Drosophila), Serine/threonine-protein kinase 13, serine/threonine-protein kinase PLK1, STPK13 |
| Gene Symbols |
PLK1 |
| Host Species |
Rabbit |
| Immunogen |
PLK1 [p Thr210] Antibody was produced by repeated immunizations with a synthetic phospho peptide corresponding to an internal region near aa 200-225 of Human Polo-like kinase 1 (Plk1) protein. (Uniprot: P53350) |
| Molecular Weight of Antigen |
68.25 kDa |
| Purification Method |
Affinity Purified |
| Quantity |
0.1 mg |
| Regulatory Status |
RUO |
| Research Discipline |
Breast Cancer, Cancer, Cell Cycle and Replication, Checkpoint signaling, Core ESC Like Genes, DNA Double Strand Break Repair, DNA Repair, Mitotic Regulators, Phospho Specific, Stem Cell Markers |
| Primary or Secondary |
Primary |
| Gene ID (Entrez) |
5347 |
| Test Specificity |
Anti-Polo-like Kinase pT210 Antibody is directed against human phosphorylated Plk1 protein. This antibody is specific for phosphorylated human PLK1 protein at the pT210 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with PLK1 homologues from human, chimpanzee, pig, chicken, mouse, rat, Xenopus species, dog, mosquito, zebra fish, starfish, sea urchin and fruit fly. Cross reactivity with PLK1 protein homologues from C.elgans and honeybee may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with PLK1 protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein. |
| Target Species |
Human, Mouse, Rat, Xenopus species, Canine, Chicken, Drosophila, Insect, Invertebrate, Primate, Zebrafish |
| Content And Storage |
Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles. |
| Isotype |
IgG |
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