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PLK1, p Thr210 Antibody, Novus Biologicals™
SDP

Catalog No. NBP178021 Shop All R&D Systems Products
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NBP178021 0.1 mg
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Catalog No. NBP178021 Supplier Novus Biologicals Supplier No. NBP178021
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Rabbit Polyclonal Antibody

PLK1 Polyclonal specifically detects PLK1 in Human, Mouse samples. It is validated for Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

Antigen PLK1 (p Thr210)
Applications Western Blot, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
Classification Polyclonal
Concentration 1 mg/ml
Conjugate Unconjugated
Dilution Western Blot 1:200-1:2000, ELISA 1:3000-1:12000, Immunohistochemistry 1:200-1:1000, Immunohistochemistry-Paraffin 1:200
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
Gene Accession No. P53350
Gene Alias cell cycle regulated protein kinase, EC 2.7.11, EC 2.7.11.21, PLK, PLK-1, polo (Drosophia)-like kinase, polo like kinase, polo-like kinase 1polo-like kinase (Drosophila), Serine/threonine-protein kinase 13, serine/threonine-protein kinase PLK1, STPK13
Gene Symbols PLK1
Host Species Rabbit
Immunogen PLK1 [p Thr210] Antibody was produced by repeated immunizations with a synthetic phospho peptide corresponding to an internal region near aa 200-225 of Human Polo-like kinase 1 (Plk1) protein. (Uniprot: P53350)
Molecular Weight of Antigen 68.25 kDa
Purification Method Affinity Purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Breast Cancer, Cancer, Cell Cycle and Replication, Checkpoint signaling, Core ESC Like Genes, DNA Double Strand Break Repair, DNA Repair, Mitotic Regulators, Phospho Specific, Stem Cell Markers
Primary or Secondary Primary
Gene ID (Entrez) 5347
Test Specificity Anti-Polo-like Kinase pT210 Antibody is directed against human phosphorylated Plk1 protein. This antibody is specific for phosphorylated human PLK1 protein at the pT210 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with PLK1 homologues from human, chimpanzee, pig, chicken, mouse, rat, Xenopus species, dog, mosquito, zebra fish, starfish, sea urchin and fruit fly. Cross reactivity with PLK1 protein homologues from C.elgans and honeybee may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with PLK1 protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.
Target Species Human, Mouse, Rat, Xenopus species, Canine, Chicken, Drosophila, Insect, Invertebrate, Primate, Zebrafish
Content And Storage Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
Isotype IgG
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