PLK1 Polyclonal Antibody, Rockland™

Catalog No.	Quantity
50-199-1302	100 μg
50-199-1303	25 μL

Catalog No. 50-199-1302 Supplier Rockland Immunochemicals Supplier No. 200901MH7

Description

Chicken Polyclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Reactivity occurs against human PLK1. A BLAST analysis was used to suggest cross reactivity with PLK1 protein from rat, mouse, and bovine sources based on 100% homology with the immunizing sequence.

PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.

Specifications

• Antigen: PLK1
• Applications: ELISA, Immunofluorescence, Western Blot
• Classification: Polyclonal
• Concentration: 1.05 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.002M sodium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7
• Gene: Plk1
• Gene Accession No.: P53350
• Gene Alias: BOS_22765; cell cycle regulated protein kinase; Plk; Plk1; PLK-1; plk1.S; plx1; polo (Drosophia)-like kinase; polo like kinase; polo like kinase 1; polo like kinase 1 S homeolog; polo-like kinase; Polo-like kinase 1; polo-like kinase homolog; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14; XELAEV_18047692mg
• Gene Symbols: Plk1
• Host Species: Chicken
• Immunogen: Anti-PLK1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to an internal portion of human PLK1 conjugated to Keyhole Limpet Hemocyanin (KLH).
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 5347
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid