Invitrogen™ PMCA3 ATPase Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1916

Catalog No. PA1916

Description

PA1-916 detects calcium pump of the plasma membrane 3 (PMCA3 ATPase) from rat, human and porcine tissues. PA1-916 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~127 kDa protein representing PMCA3a and PMCA3b ATPase from rat brain microsomal fractions. PA1-916 immunizing peptide corresponds to amino acid residues 5-22 from rat PMCA3 ATPase protein. This sequence is completely conserved between rat and human PMCA3 ATPase.

The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified.

Specifications

• Antigen: PMCA3 ATPase
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ATP2B3
• Gene Alias: 6430519O13Rik; ATP2B3; ATPase plasma membrane Ca2+ transporting 3; ATPase, Ca++ transporting, plasma membrane 3; CFAP39; cilia and flagella associated protein 39; CLA2; LOW QUALITY PROTEIN: plasma membrane calcium-transporting ATPase 3; OPCA; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 3; plasma membrane calcium pump; Plasma membrane calcium pump isoform 3; Plasma membrane calcium-transporting ATPase 3; PMCA3; PMCA3a; SCAX1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100520061, 29599, 492
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: Q16720, Q64568
• Gene Symbols: ATP2B3
• Immunogen: Synthetic Peptide: A(5) N S S I E F H P K P Q Q Q R E V P(22).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Rat, Pig
• Product Type: Antibody
• Isotype: IgG