PMCA4 ATPase Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA5111763

Catalog No. PIPA5111763

Description

Reconstitute antibody with 50 μL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min). Peptide sequence is: CDRILNKEGG IVPFKTK Homology: Mouse - 12/17 amino acid residues identical.

The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin (Cat. # MA3-917 and MA3-918).

Specifications

• Antigen: PMCA4 ATPase
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Atp2b4
• Gene Alias: ATP2B2; Atp2b4; BOS_15586; Matrix-remodeling-associated protein 1; MXRA1; Plasma membrane calcium ATPase isoform 4; Plasma membrane calcium pump isoform 4; Plasma membrane calcium-transporting ATPase 4; PMCA4; PMCA4b; PMCA4x
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 29600
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Western Blot
• Concentration: 0.8 mg/mL
• Formulation: PBS with 1% BSA and 0.05% sodium azide; pH 7.4
• Gene Accession No.: Q64542
• Gene Symbols: Atp2b4
• Immunogen: Peptide corresponding to amino acid residues 601-617 of rat PMCA4 (2nd intracellular loop)
• Quantity: 50 μL
• Primary or Secondary: Primary
• Target Species: Rat
• Product Type: Antibody
• Isotype: IgG