MilliporeSigma™ PML Isoform II, Mouse, Unlabeled, Clone: 1A8.1,

Catalog No.	Quantity	Conjugate
MABE175MI	100 μg	Unconjugated

Catalog No. MABE175MI Supplier MilliporeSigma™ Supplier No. MABE175

Description

Mouse Monoclonal Antibody

Protein PML (UniProt P29590; also known as Promyelocytic leukemia protein, RING finger protein 71, Tripartite motif-containing protein 19) is encoded by the PML (also known as MYL, PP8675, RNF71, TRIM19) gene (Gene ID 5371) in human. The human PML gene/protein adopted its name when it was originally discovered as an RARα fusion protein caused by chromosomal translocation in acute promyelocytic leukemia (APL) patients. In normal cells, the PML protein interacts with multiple protein partners in the nucleus to form PML nuclear bodies (PML-NBs) that mediate a variety of cellular functions including senescence, apoptosis, protein degradation, telemere stasbility, and antiviral defense. At least 12 PML isoforms are generated by alternative splicings, including six nuclear isoforms (PML-I to PML-VI) and the cytoplasmic isoform PML-VII (a.k.a. PML-VIIB), other spliced isoforms wihtout exon 6-coding sequence (a.a. 467-552) are likewise expected to be cytoplasmic due to the abscence of nuclear localization signal (NLS) encoded by exon 6. All isoforms share the same N-terminal region (a.a. 1-419) containing the RBCC/tripartite motif and may have related functions due to their common functional RBCC/TRIM domain, while increasing evidences also suggest that each PML isoform possesses distinct functions mediated by its specific C-terminal sequence. Isoform PML-1 (a.k.a. PML-I, TRIM19alpha; UniProt P29590-1) and PML-2 (a.k.a. PML-II or TRIM19kappa; UniProt P29590-8) share the same N-terminal 570-a.a. sequence (encoded by exons 1 to 7a), however PML-1 derives its remaining C-terminal sequence (a.a. 571-882) from exons 8a and 9, while PML-II derives its (a.a. 571-829) from exon 7b with a different reading frame as that of PML-V.

Specifications

• Antigen: PML Isoform II
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 1A8.1
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: PML, MYL, PP8675, RNF71, TRIM19
• Gene Accession No.: P29590-8
• Host Species: Mouse
• Immunogen: KLH-conjugated linear peptide corresponding to a C-terminal sequence of human PML Isoform II.
• Purification Method: Protein G Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Epigenetics & Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_150242
• Test Specificity: Clone 1A8.1 was raised against an C-terminal region sequence unique to PML isoform II (PML-2), but not present in the other 11 PML spliced isoforms reported by UniProt (P29590).
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only. Not for use in diagnostic procedures.