Invitrogen™ PNPase Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA522397

Catalog No. PIPA522397

Description

Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (92%), Rat (93%), Pig (93%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

PNPT1 (polyribonucleotide nucleotidyltransferase 1, mitochondirla) is a RNA-binding protein implicated in numerous RNA metabolic processes. It catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. PNPT1 is a component of the mitochondrial degradosome (mtEXO) complex that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. It is required for correct processing and polyadenylation of mitochondrial mRNAs. It plays a role as a cytoplasmic RNA import factor, in mitochondrial morphogenesis and respiration, regulation of expression of the electron transport chain, regulation of the stability of specific mature miRNAs in melanoma cells, and RNA cell surveilance. Mutations of the gene can result in combined oxidative phosphorylation deficiency 13 (COXPD13) and deafness, autosomal recessive, 70 (DFNB70).

Specifications

• Antigen: PNPase
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PNPT1
• Gene Alias: 1200003F12Rik; 3'-5' RNA exonuclease OLD35; COXPD13; DFNB70; DKFZp762K1914; old 35; Old35; old-35; PNPase; PNPase 1; PNPase old-35; Pnpt1; Pnptl1; polynucleotide phosphorylase 1; Polynucleotide phosphorylase-like protein; polyribonucleotide nucleotidyltransferase 1; polyribonucleotide nucleotidyltransferase 1, mitochondrial
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 87178
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q8TCS8
• Gene Symbols: PNPT1
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 783 of PNPase (Uniprot ID#Q8TCS8).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG