Podoplanin, Mouse anti-Human, Clone: LpMab-13, Millipore Sigma™

Catalog No.	Quantity
MABT85525UG	25 μg

Catalog No. MABT85525UG Supplier MilliporeSigma Supplier No. MABT85525UG

Description

Mouse Monoclonal Antibody

Anti-Podoplanin, clone LpMab-13, Cat. No. MABT855, is a mouse monoclonal antibody that detects human podoplanin and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.

Podoplanin (UniProt: Q86YL7; also known as Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A) is encoded by the PDPN (also known as GP36) gene (Gene ID: 10630) in human. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Six isoforms of podoplanin have been described that are produced by alternative splicing. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-131), a helical domain (aa 132-152) and a cytoplasmic region (aa 153-162). Podoplanin possesses three tandem repeat of platelet aggregation-stimulating (PLAG) domains in its N-terminus. Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2). Interaction of human podoplanin with CLEC-2 mainly involves Glu47 and Asp48 in the platelet PLAG3. Clone LpMab-13 binds to PLAG2/3 domains and recognizes endogenous hPDPN of cancer cells, including glioblastoma, oral cancer, lung cancer, and malignant mesothelioma, and normal cells such as lymphatic endothelial cells and podocytes of kidney. The interaction between LpMab-13 and hPDPN is independent of its glycosylation. The minimum epitope of LpMab-13 is identified to be Ala42-Asp49 of hPDPN. ((Ref.: Ogasawara, S., et al. (2016). Monoclon. Antib. immunodiag. Immunother. 35(3); 155-163).

Specifications

• Antigen: Podoplanin
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: LpMab-13
• Conjugate: Unconjugated
• Dilution: ELISA Analysis: A representative lot detected Podoplanin in ELISA application (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(3):155-62). Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry application (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(3):155-62). Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry application (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(3):155-62). Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting application (Ogasawara, S., et. al. (2016). Monoclon Antib Immunodiagn Immunother . 35(3):155-62).
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A
• Gene Symbols: PDPN;GP36;PSEC0003;PSEC0025
• Host Species: Mouse
• Immunogen: LN229 glioma cells expressing human Podoplanin.
• Purification Method: Protein G purified
• Quantity: 25 μg
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Test Specificity: Clone LpMab-13 detects Podoplanin in human cells. The minimal epitope is shown to be Ala42-Asp49.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG1