Podoplanin, Mouse anti-Monkey, Human, Clone: LpMab-12, Millipore Sigma™

Catalog No.	Quantity
MABT84125UG	25 μg

Catalog No. MABT84125UG Supplier MilliporeSigma Supplier No. MABT84125UG

Description

Mouse Monoclonal Antibody

Anti-Podoplanin, clone LpMab-12, Cat. No. MABT841, is a mouse monoclonal antibody that detects human and monkey podoplanin and has been tested for use in ELISA, Flow Cytometry, and Immunohistochemistry (Paraffin).

Podoplanin (UniProt: Q86YL7; also known as Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A) is encoded by the PDPN (also known as GP36) gene (Gene ID: 10630) in human. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Six isoforms of podoplanin have been described that are produced by alternative splicing. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-131), a helical domain (aa 132-152) and a cytoplasmic region (aa 153-162). Podoplanin possesses three tandem repeat of platelet aggregation-stimulating (PLAG) domains in its N-terminus. Among the PLAG domains, sialylated O-glycan on Thr52 of PLAG3 is essential for the binding to C-type lectin-like receptor-2 (CLEC-2). Interaction of human podoplanin with CLEC-2 mainly involves Glu47 and Asp48 in the platelet PLAG3. Clone LpMab-12 specifically detects sialylated O-Glycan on Thr52 of platelet aggregation-stimulating domain of human podoplanin. It also reacts with the synthetic glycopeptide of hPDPN, corresponding to 38 54 amino acids that carries 2-6 sialylated N-acetyl-D-galactosamine (GalNAc) on Thr52. The minimal epitope of LpMab-12 is identified as Asp49 Pro53 of hPDPN. (Ref.: Kato, Y et al. (2016). PLoS ONE 11(3): e0152912).

Specifications

• Antigen: Podoplanin
• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin)
• Classification: Monoclonal
• Clone: LpMab-12
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Podoplanin in human testis cancer tissue.ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Kato, Y., et. al. (2016). PLoS One. 11(3):e0152912).
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Aggrus;Glycoprotein 36;Gp36;PA2.26 antigen;T1-alpha;T1A
• Gene Symbols: PDPN;GP36;PSEC0003;PSEC0025
• Host Species: Mouse
• Immunogen: LN229 glioma cells expressing human Podoplanin.
• Purification Method: Protein G purified
• Quantity: 25 μg
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Test Specificity: Clone LpMab-12 detects Podoplanin in human and monkey cells. It targets an epitope within the N-terminal half. It specifically detects Sialylated O-Glycan on Thr52 of platelet aggregation-stimulating domain of human podoplanin. The minimal epitope of LpMab-12 is identified as Asp49-Pro53.
• Target Species: Monkey, Human
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG1 κ