Podoplanin, Mouse anti-Rat, Clone: PMab-2, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABT85025UG

Catalog No. MABT85025UG

Description

Anti-Podoplanin, clone PMab-2, Cat. No. MABT850, is a mouse monoclonal that detects rat podoplanin and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting.

Podoplanin (UniProt: Q64294; also known as E11 antigen epitope, Glycoprotein 38, Gp38, OTS-8, RTI140, T1-alpha, T1A, Type I cell 40 kDa protein) is encoded by the Pdpn (also known as Gp38, Ots8) gene (Gene ID: 54320) in rat. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells. It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. It is synthesized with a signal peptide of 22 amino acids, which is cleaved to produce the mature form. Podoplanin contains an extracellular domain (aa 23-135), a helical domain (aa 136-156) and a cytoplasmic region (aa 157-166). Podoplanin is extensively O-glycosylated and contains sialic acid residues. O-glycosylation is believed to be essential for its platelet aggregation activity. Clone PMab2 exhibits high affinity for podoplanin (Kd = 0.59 nM). (Ref.: Oki, H et al. (2015). Monoclon. Antib. Immunodiagn. Immunother. 34(6); 396-403).

Specifications

• Antigen: Podoplanin
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: Pdpn;Gp38;Ots8
• Immunogen: KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of rat Podopalnin.
• Quantity: 25 μg
• Research Discipline: Cell Structure
• Test Specificity: Clone PMab-2 detects podoplanin in rat spleen and kidney. It targets an epitope within 14 amino acids from the N-terminal region.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG1 κ

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Clone: PMab-2
• Dilution: Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in rat spleen tissue. Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Oki, H., et. al. (2015).   Monoclon Antib Immunodiagn Immunother . 34(6):396-403). Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Oki, H., et. al. (2015).   Monoclon Antib Immunodiagn Immunother . 34(6):396-403). Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Oki, H., et. al. (2015).   Monoclon Antib Immunodiagn Immunother . 34(6):396-403). ELISA Analysis: A representative lot detected Podoplanin in ELISA applications (Oki, H., et. al. (2015).   Monoclon Antib Immunodiagn Immunother . 34(6):396-403).
• Gene Alias: E11 antigen epitope;Glycoprotein 38;Gp38;OTS-8;RTI140;T1-alpha;T1A;Type I cell 40 kDa protein
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Rat
• Form: Purified