Podoplanin, Rat anti-Mouse, Clone: PMab-1, Millipore Sigma™

Catalog No.	Quantity
MABT85125UG	25 μg

Catalog No. MABT85125UG Supplier MilliporeSigma Supplier No. MABT85125UG

Description

Rat Monoclonal Antibody

Anti-Podoplanin, clone PMab-1, Cat. No. MABT851, is a rat monoclonal antibody that detects mouse Podoplanin and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Podoplanin (UniProt: Q62011; also known as Aggrus, Glycoprotein 38, Gp38, OTS-8, PA2.26 antigen, T1-alpha, T1A, Transmembrane glycoprotein E11) I encoded by the Pdpn (also known as Gp38, Ots8) gene (Gene ID: 14726) in murine species. Podoplanin serves as the endogenous ligand of C-type lectin-like receptor-2 (CLEC-2) and is highly expressed in various tumors and in some normal cells, such as lymphatic endothelial cells and podocytes. Podoplanin is detected at high levels in lung and brain, at lower levels in kidney, stomach, liver, spleen and esophagus, and not detected in skin and small intestine. It is expressed in epithelial cells of choroid plexus, ependyma, glomerulus and alveolus, in mesothelial cells and in endothelia of lymphatic vessels. It is also expressed in stromal cells of peripheral lymphoid tissue and thymic epithelial cells and is detected in carcinoma cell lines and cultured fibroblasts. High expression of Podoplanin is observed in colon carcinomas. . It may be involved in cell migration and/or actin cytoskeleton organization. Podoplanin is localized to actin-rich microvilli and plasma membrane projections, such as filopodia, lamellipodia, and ruffles. Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. Podoplanin levels are down-regulated by treatment with puromycin aminonucleoside. Mice lacking or with defective podoplanin die at birth of respiratory failure due to a low number of attenuated type I cells, narrow and irregular air spaces, and defective formation of alveolar saccules. Administration of PMab-1 antibody is shown to reduce lymphangiogenesis in the corneal suture and ear wound healing murine models. It suppresses thioglycollate-induced macrophages infiltration at the site of wound healing. (Ref.: Maruyama, Y et al (2014). Invest.Ophthal. Visual Sci. 55(8); 4813-4822).

Specifications

• Antigen: Podoplanin
• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: PMab-1
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Podoplanin in mouse lung tissue. Immunohistochemistry Analysis: A representative lot detected Podoplanin in Immunohistochemistry applications (Kaji, C., et. al. (2012) Acta Histochem Cytochem . 45(4):227-37). Flow Cytometry Analysis: A representative lot detected Podoplanin in Flow Cytometry applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother . 34(6):396-403). Western Blotting Analysis: A representative lot detected Podoplanin in Western Blotting applications (Oki, H., et. al. (2015). Monoclon Antib Immunodiagn Immunother . 34(6):396-403). Immunocytochemistry Analysis: A representative lot detected Podoplanin in Immunocytochemistry applications (Kaji, C., et. al. (2012) Acta Histochem Cytochem . 45(4):227-37).
• Formulation: Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Alias: Aggrus;Glycoprotein 38;Gp38;OTS-8;PA2.26 antigen;T1-alpha;T1A;Transmembrane glycoprotein E11
• Gene Symbols: Pdpn;Gp38;Ots8
• Host Species: Rat
• Immunogen: KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of murine Podoplanin.
• Purification Method: Protein G purified
• Quantity: 25 μg
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Test Specificity: Clone PMab-1 detects murine Podoplanin. It targets an epitipe within 14 amino acids from the N-terminal region.
• Target Species: Mouse
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Form: Purified
• Isotype: IgG2a κ