Thermo Scientific™ PolarScreen™ ER Alpha Competitor Assay, Green

Catalog No.	Quantity
A15882	2500 Assays
A15883	800 Assays

Catalog No. A15882 Supplier Thermo Scientific™ Supplier No. A15882

Description

The PolarScreen™ ER (Estrogen Receptor) Alpha Competitor Assay Kit, Green, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential ER alpha ligands. The kit uses insect cell-expressed, full-length, native (untagged) human estrogen receptor alpha (available separately, Cat. No. A15674) and a novel, high-affinity, fluorescent estrogen ligand (Fluormone ES2 Green) in a homogenous, mix-and-read assay format.

This kit produces a green color read-out; a 'red' kit is also available. Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation. For additional information or assistance contact drugdiscoverytech@lifetech.com.

PolarScreen™ ER Alpha Competitor Assay, Green, is:

Complete & ready-to-use out of the box—all reagents included, just add your test compounds

High throughput compatible—800 x 20μL fluorescence polarization assays in a 384-well plate

Convenient—available in bulk sizes for larger screening needs

How the Assay Works
Full length ER alpha is added to a fluorescent estrogen ligand to form an ER-Fluormone ES2 complex. This complex is added to individual test compounds in multiwell plates. To determine relative affinity, the assay measures fluorescence polarization. Competing test compounds will displace the Fluormone ES2 ligand from ER alpha, permitting it to tumble rapidly and resulting in low polarization values. A shift in the fluorescence polarization value in the presence of test compound is used to determine the relative affinity of test compounds for ER alpha.

All components are manufactured under the strictest quality parameters. This product's performance, per the Certificate of Analysis, is guaranteed for 6 months after purchase. Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.

Specifications

• Content And Storage: Contains:
  
  • 4 tubes full-length ER Alpha Recombinant Protein (store at -68 to -85°C)
  • 1 tube of Fluormone ES2 Green Tracer (store at -5 to -30°C)
  • 2 bottles of ES2 Screening Buffer (store at -5 to -30°C)
• Detection Method: Fluorescence
• For Use With (Equipment): Fluorescent Imager
• Format: 384-well Plate
• Quantity: 2500 Assays
• Type: Competitor Assay Kit

Frequently Asked Questions (FAQs)

I'm using the TaqMan hPSC Scorecard Panel. How do I set up my experimental run?

The template files contain all of the necessary experimental details for you to run your instrument. There is no need to modify any of the experimental details if you are using the template file (.edt). Step-by-step guides on how to run the qPCR instruments are available here.

For Research Use Only. Not for use in diagnostic procedures.