POLB Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 600401C65S

Catalog No. 50-199-2030

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antibody reacts with overexpressed and endogenous POL beta protein. A BLAST analysis was used to suggest reactivity with POL beta from human, bovine, and Xenopus laevis based on a 100% homology with the immunizing sequence. Cross-reactivity with POL beta from other sources has not been determined.

DNA polymerase beta comprises an amino-terminal 8- kDa domain and a carboxy-terminal 31- kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

Specifications

• Antigen: POLB
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: POLB
• Gene Alias: A430088C08Rik; BOS_23903; DNA pol beta; DNA polymerase beta; DNA polymerase beta subunit; DNA-directed DNA polymerase beta; LOW QUALITY PROTEIN: DNA polymerase beta; MGC125976; POL B; Pol beta; POLB; polb.S; polymerase (DNA directed), beta; polymerase (DNA directed), beta S homeolog; polymerase (DNA) beta; polymerase B; polymerase-beta; wu:fa97a05; XELAEV_18020165mg; zgc:109924
• Host Species: Rabbit
• Regulatory Status: RUO
• Gene ID (Entrez): 5423
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P06746
• Gene Symbols: POLB
• Quantity: 25 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody