Invitrogen™ POLE2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA536952

Catalog No. PIPA536952

Description

This antibody detects endogenous protein at a molecular weight of 60 kDa. Purity is >95% by SDS-PAGE.

DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. Exonucleases involved in these processes include DNA polymerases, including DNA pol delta and epsilon. DNA pol delta consists of two subunits-p125 which interacts directly with the sliding DNA clamp protein PCNA, and p50. DNA pol delta can be regulated by cell cycle proteins. DNA pol epsilon is a multiple subunit enzyme, the catalytic subunit of which is encoded by the POL2 gene. The exact reactions catalyzed by DNA pol delta and epsilon on leading and lagging strands have not yet been elucidated.

Specifications

• Antigen: POLE2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: POLE2
• Gene Alias: DNA pol epsilon2; DNA pol epsilonB; DNA polymerase epsilon 2, accessory subunit; DNA polymerase epsilon small subunit; DNA polymerase epsilon subunit 2; DNA polymerase epsilon subunit B; DNA polymerase epsilon, subunit 2; DNA polymerase II subunit 2; DNApol epsilon2; DNApol epsilonB; DPE2; POLE2; polymerase (DNA directed), epsilon 2 (p59 subunit); polymerase (DNA directed), epsilon 2, accessory subunit; polymerase (DNA) epsilon 2, accessory subunit
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18974, 299112, 5427
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: O54956, P56282
• Gene Symbols: POLE2
• Immunogen: Synthetic peptide corresponding to amino acids 144-189 of human DNA pol epsilon 2.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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