Invitrogen™ POLI Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA316539

Catalog No. APSA6539

Description

Suggested positive control: HeLa whole cell extract (100 μg) or GM0193 (lymphoblastoid cell line)..

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

Specifications

• Antigen: POLI
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Poli
• Gene Alias: DNA polymerase iota; eta2; Poli; polymerase (DNA directed) iota; polymerase (DNA directed), iota; polymerase (DNA) iota; polymerase (DNA-directed), iota; RAD 30B; RAD30; RAD30 homolog B; RAD30B; RAD3OB
• Host Species: Rabbit
• Quantity: 50 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot, Immunocytochemistry
• Concentration: Conc. Not Determined
• Formulation: Whole serum with 0.02% sodium azide
• Gene Accession No.: Q6R3M4, Q9UNA4
• Gene Symbols: Poli
• Immunogen: A 15-mer peptide in the C-terminal region of human DNA Polymerase Iota, conjugated to KLH.
• Regulatory Status: RUO
• Gene ID (Entrez): 11201, 26447
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov