Polo-like kinase-1, Rabbit anti-Mouse, Human, Polyclonal, Millipore Sigma™

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma ABE261925UG

Catalog No. ABE261925UG

Description

Anti-Polo-like kinase-1, Cat. No. ABE2619, is a rabbit polyclonal antibody that detects Polo-Like Kinse 1 and has been tested for use Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Serine/threonine-protein kinase PLK1 (UniProt: P53350; also known as EC: 2.7.11.21, Polo-like kinase 1, PLK-1, Serine/threonine-protein kinase 13, STPK13) is encoded by the PLK1 (also known as PLK) gene (Gene ID: 5347) in human. PLK-1 is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. It is shown to express in placenta and colon and its activity is enhanced by growth promoting agents. PLK-1 is activated by phosphorylation at Thr210 by aurora kinase A. It accumulates to a maximum during the G2 and M phases and declines to a nearly undetectable levels following mitosis and throughout G1 phase. However, it begins to accumulate again during the S phase of cell cycle. The kinase domain of PLK-1 is localized to amino acids 53-305 with activation loop in the region of amino acids 194-221. PLK-1 contains 2 POLO box domains (aa 417-480 and 515-584), which act as phosphopeptide-binding module that recognize and bind serine-phosphothreonine/ phosphoserine]-(proline/X) motifs. PLK-1 recognizes and binds docking proteins that are already phosphorylated on these motifs, and then phosphorylates them. Defects in PLK1 gene are associated with some cancers, such as gastric, thyroid or B-cell lymphomas. Expression is shown to be higher in tumor tissues with a poor prognosis, suggesting its role in malignant transformations and carcinogenesis.

Specifications

• Antigen: Polo-like kinase-1
• Classification: Polyclonal
• Dilution: Immunofluorescence Analysis: A representative lot detected Polo-like kinase-1 in U2OS cells (Macurek, L., et. al. (2008). Nature. 455(7209):119-23).Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Polo-like kinase-1 in U2OS and C2C12 cells.Western Blotting Analysis: A representative lot detected Polo-like kinase-1 in U2OS-TR transfected cells (Macurek, L., et. al. (2008). Nature. 455(7209):119-23).Immunoprecipitation Analysis: A representative lot detected Polo-like kinase-1 in U2OS-TR transfected cells (Macurek, L., et. al. (2008). Nature. 455(7209):119-23).
• Gene Alias: Serine/threonine-protein kinase PLK1;EC: 2.7.11.21;PLK-1;Serine/threonine-protein kinase 13;STPK13
• Host Species: Rabbit
• Molecular Weight of Antigen: ∽68 kDa observed; 68.26 kD calculated. Uncharacterized bands may be observed in some lysate(s).
• Quantity: 25 μg
• Research Discipline: Signaling
• Test Specificity: This rabbit polyclonal antibody detects human Polo-like kinase 1. It targets an epitope within 303 amino acids from the C-terminal half.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG

• Applications: Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in PBS containing 80 mM Glycine with 0.1% sodium azide.
• Gene Symbols: PLK1;PLK
• Immunogen: His-tagged recombinant fragment corresponding to 303 amino acids from the C-terminal half of human Polo-like kinase-1.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse, Human
• Form: Purified