Invitrogen™ POLR1A Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA556766

Catalog No. PIPA556766

Description

Immunogen sequence: SNYEVIMKSQ HLHEVLSRAD PKKALHHFRA IKKWQSKHPN TLLRRGAFLS YSQKIQEAVK ALKLESENRN GRSPGTQEML RMWYELDEES RRKYQ Highest antigen sequence identity to the following orthologs: Mouse - 73%, Rat - 76%.

SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T cell lines or mice have provided strong evidence that SLP-76 plays a positive role in promoting T cell development and activation as well as mast cell and platelet function.

Specifications

• Antigen: POLR1A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: POLR1A
• Gene Alias: 194kDa; 3010014K16Rik; A190; A194; AFDCIN; DNA-directed RNA polymerase I A; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A; DNA-directed RNA polymerase I subunit A1; DNA-directed RNA polymerase I subunit RPA1; mRPA1; Polr1a; polymerase (RNA) I polypeptide A; polymerase (RNA) I polypeptide A, 194kDa; polymerase (RNA) I subunit A; RNA polymerase 1-4; RNA polymerase 1-4 (194 kDa subunit); RNA polymerase I (194 kDa subunit); RNA polymerase I 194 kDa subunit; RNA polymerase I subunit A; RNA polymerase I subunit A1; Rpa1; RPA194; RPO14; RPO1-4
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 25885
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ChIP Assay, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.2 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: O95602
• Gene Symbols: POLR1A
• Immunogen: Recombinant protein corresponding to Human POLR1A. Recombinant protein control fragment (Product #RP-95969).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG