Invitrogen™ POLR2I Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA541120

Catalog No. PIPA541120

Description

Peptide sequence: ACRNCDYQQE ADNSCIYVNK ITHEVDELTQ IIADVSQDPT LPRTEDHPCQ Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 83%; Zebrafish: 93%.

POLR2I is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. POLR2I has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests. This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity.

Specifications

• Antigen: POLR2I
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: POLR2I
• Gene Alias: 2810002B19Rik; DNA-directed RNA polymerase II 14.5 kDa polypeptide; DNA-directed RNA polymerase II subunit I; DNA-directed RNA polymerase II subunit RPB9; hRPB14.5; Polr2i; polymerase (RNA) II (DNA directed) polypeptide I; polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa; polymerase (RNA) II subunit I; RNA polymerase II 14.5 kDa subunit; RNA polymerase II subunit B9; RNA polymerase II subunit I; RPB14.5; RPB9; Unknown (protein for MGC:133488); zgc:103515
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 5438
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.5 mg/mL
• Formulation: PBS with 2% sucrose and 0.09% sodium azide
• Gene Accession No.: P36954
• Gene Symbols: POLR2I
• Immunogen: Synthetic peptide directed towards the N-terminal of human POLR2I (aa 38-87).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG