Invitrogen™ POLR3F Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA528314

Catalog No. PIPA528314

Description

Recommended positive controls: HeLa nucleus. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (91%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The human POLR3F is a component of RNA III polymerase. RNA polymerase III transcribes many essential, small, noncoding RNAs, including the 5S rRNAs and tRNAs. While most pol III-transcribed genes are found scattered throughout the linear chromosome maps or in multiple linear clusters, there is increasing evidence that many of these genes prefer to be spatially clustered, often at or near the nucleolus. This association could create an environment that fosters the coregulation of transcription by pol III with transcription of the large ribosomal RNA repeats by RNA polymerase I (pol I) within the nucleolus. Given the high number of pol III-transcribed genes in all eukaryotic genomes, the spatial organization of these genes is likely to affect a large portion of the other genes in a genome. POLR3F has also been recently identified as an HIV dependency factor (HDF), suggesting that POLR3F may be an important drug target in HIV treatment. At least two isoforms of POLR3F are known to exist.

Specifications

• Antigen: POLR3F
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: POLR3F
• Gene Alias: 2810411G20Rik; 3010019O03Rik; 3110032A07Rik; DNA-directed RNA polymerase III subunit F; DNA-directed RNA polymerase III subunit RPC6; Polr3f; polymerase (RNA) III (DNA directed) polypeptide F; polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa; polymerase (RNA) III subunit F; RNA polymerase III 39 kDa subunit; RNA polymerase III C39 subunit; RNA polymerase III subunit C6; RNA polymerase III subunit F; RPC39; RPC6
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10621
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9H1D9
• Gene Symbols: POLR3F
• Immunogen: Recombinant protein encompassing a sequence within the center region of human POLR3F. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG