Poly-D-Lysine Hydrobromide, MP Biomedicals™

Description

Enhances cell adhesion to solid substrates. Poly-D-Lysine Hydrobromide, MP Biomedicals is a synthetic amino acid that binds to DNA, red cell membrane and any negatively charged protein. It has been shown to eliminate prion proteins from infected cells. Mo

When adsorbed to the culture surface, poly-lysine increases the number of positively charged sites available for cell binding. It is a compromise between easier-to-use lower molecular weight products and the extremely viscous higher molecular weights.

Poly-lysine enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. Both the D- and L- forms can be used as a coating substrate, since poly-lysine is a nonspecific attachment factor for cells; however, certain cells can digest it. In this case, poly-D-lysine should be used as the attachment factor so that the cells are not disrupted by excessive uptake of L-lysine.

Poly-D-Lysine Hydrobromide is typically used as a coating substrate for culture dishes, slides, etc. Other uses for poly-lysine have been reported as well:

• Conjugation to methotrexate for increased drug transport
• Microencapsulation of islets
• Use in simple reproducible procedure for chromosomal preparations from a variety of tissues
• Immobilation of living cells in biocompatible semipermeable microcapsules
• In the preparation of polycationic beads
• Conjugation to albumin and horseradish peroxidase to enhance cellular uptake

Specifications

Chemical Identifiers

• CAS: 27964-99-4
• Molecular Formula: C6H15BrN2O2
• Molecular Weight (g/mol): 227.102
• InChI Key: MEXAGTSTSPYCEP-NUBCRITNSA-N
• Synonym: d-lysine hydrobromide
• PubChem CID: 87493163
• IUPAC Name: (2R)-2,6-diaminohexanoic acid;hydrobromide
• SMILES: C(CCN)CC(C(=O)O)N.Br

• Solubility Information: 50mg/mL, H2O
• Quantity: 100 mg
• Formula Weight: 70000-150000
• Physical Form: Powder

Safety and Handling

Recommended Storage

Store at -20°C.