Thermo Scientific™ POROS™ A 20 μm Protein A Affinity HPLC Columns

Catalog No.	Material	Dimensions	Quantity
15-022-24	PEEK	4.6 x 50 mm	1 Column
15-022-26	PEEK	4.6 x 100 mm	1 Column
15-024-12	Stainless Steel	2.1 x 30 mm	1 Column
21-001-00A	PEEK	2.1 x 30 mm	1 Column: 2.1 x 30 mm

Catalog No. 15-022-24 Supplier Thermo Scientific™ Supplier No. 1502224

Description

Thermo Scientific™ POROS™ Protein A is based on an immobilized recombinant Protein A functional group designed for high throughput purification of antibodies. The 20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS Protein A is based on an immobilized recombinant Protein A functional group designed for high throughput purification of antibodies. The 20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS High Performance Chromatography™ Media and Pre-Packed Columns

• A high performance chromatography resins for analytical to process scale separations.
• Higher productivity: High throughput and high dynamic capacity associated with High Performance Chromatography™
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10,000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of High Performance Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed High Performance Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

High Performance Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High Capacity, High Resolution, High Speed

In contrast to conventional chromatography media, POROS High Performance Chromatography™ resins particles, are engineered to have two discreet classes of pores. Large 'throughpores' allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short 'diffusive' pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1000 to 5000 cm/hr compared to 50 to 360 cm/hr for conventional media.

Specifications

• Column Format: Analytical Column, Biocompatible PEEK
• Diameter (Metric): 4.6 mm
• Length (Metric): 50 mm
• Max. Pressure: 2400 psi (170 bar)
• Particle Size: 20 μm
• pH: 2-10
• Pore Size: 2000 Å
• Quantity: 1 Column
• Temperature: 2-40°C
• Stationary Phase: Recombinant Protein A
• Content And Storage: Store refrigerated at 2°C to 8°C
• Product Line: POROS
• Binding Capacity: 19 mg/mL
• Shipping Condition: Room Temperature
• Volume (Metric): 0.8 mL
• Dimensions: 4.6 x 50 mm
• Packing Material: Highly Crosslinked Styrene-Divinylbenzene polymer (ST-DVB)
• Column Type: Affinity
• Material: PEEK
• Technique: Bio-LC

For Research Use Only. Not for use in diagnostic procedures.