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  6. Thermo Scientific™ POROS™ CaptureSelect™ FibXL Affinity Matrix

Thermo Scientific™ POROS™ CaptureSelect™ FibXL Affinity Matrix

Catalog No. 2802912010
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Quantity:
5 mL
10 mL
50 mL

Description

Thermo Scientific™ POROS™ CaptureSelect™ FibXL Affinity Matrix has specifically been designed for the purification of human fibrinogen from recombinant or plasma source.

POROS CaptureSelect FibXL Affinity Matrix has specifically been designed for the purification of human fibrinogen from recombinant or plasma source.

Features of this affinity matrix include:

  • Fibrinogen purification from human plasma and recombinant sources
  • Efficient purification of fibrinogen with high yields and retained activity
  • Excellent scalability
  • Non-animal derived
  • High flow rates

POROS CaptureSelect FibXL Affinity Matrix is an affinity matrix that binds to fibrinogen with high selectivity and yield, enabling a robust, fast, and efficient purification process with excellent purity obtained in one step.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

  • Matrix: cross-linked poly(styrene-divinylbenzene)
  • Average particle size: 50 μm
  • Ligand: POROS CaptureSelect FibXL affinity ligand
  • Ligand coupling method: epoxide
  • Binding capacity: approximately 15 g/L resin (determined with plasma derived fibrinogen)
  • Elution conditions: acidic elution with 0.1 M glycine pH 3.0; neutral pH elution with 20 mM Tris, 1.0 M MgCl2 and 40% (v/v) propyleneglycol
  • Mechanical resistance: 100 bar (1450 psi, 10 MPa)
  • Formulation buffer: 20 % (v/v) ethanol

Specifications

Binding Capacity 15 g/L
Matrix Cross-linked poly(styrene-divinylbenzene)
Content And Storage Store in refrigerator (2°C to 8°C).
Shipping Condition Approved for shipment at Room Temperature or on Wet Ice
Particle Size 50 μm
Form Liquid Suspension
Product Line CaptureSelect™
Quantity 10 mL
Type Affinity Matrix
Ligand Type FibXL affinity ligand
Stationary Phase FIB-XL
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