Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Catalog Number	Mfr. No.	Quantity
A25741	Applied Biosystems™ A25741	1 mL
A25742	Applied Biosystems™ A25742	5 mL
A25743	Applied Biosystems™ A25743	50 mL
A25778	Applied Biosystems™ A25778	10 x 5 mL
A25780	Applied Biosystems™ A25780	5 x 1 mL
A25918	Applied Biosystems™ A25918	10 x 1 mL
A25776	Applied Biosystems™ A25776	2 x 5 mL
A25777	Applied Biosystems™ A25777	5 x 5 mL
A25779	Applied Biosystems™ A25779	2 x 1 mL

Description

Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplify targets for accurate gene expression analysis.

• A dual hot-start mechanism for excellent specificity
• Highly reproducible CTs over a broad dynamic range
• Inclusion of UDG to help prevent carryover contamination
• Stability of pre-assembled reactions for up to 72 hours
• Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism:

• Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding
• PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures
• In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification

Tight reproducibility in CTs over a wide dynamic range:

• PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested
• In addition, this master mix provides efficient amplification over 6 logs of sample input
• Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality

UDG included for carryover contamination control:

• The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives

Stability of pre-assembled reactions:

• The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data
• This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1

Broad instrument compatibility:

• PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2
• It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
• For optimal results, recommended primer concentrations are 300 to 800nM

Order Info

Shipping Conditions: Wet Ice

Specifications

• Detection Method: SYBR
• PCR Method: qPCR
• Reaction Speed: Fast or Standard
• Product Line: PowerUP™, SYBR™
• Shipping Condition: Wet Ice
• Concentration: 2X

• Format: Tube
• Polymerase: Dual-Lock Taq DNA Polymerase
• For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System
• Product Type: Real Time PCR SYBR Master Mix
• For Use With (Application): Gene Expression
• Sample Type: DNA

For Research Use Only. Not for use in diagnostic procedures.