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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

$110.00 - $4050.00

Specifications

Detection Method SYBR
Format Tube
PCR Method qPCR
Polymerase Dual-Lock Taq DNA Polymerase
Reaction Speed Fast or Standard
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Products 9
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
A25741
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Applied Biosystems™
A25741
1 mL
Each for $110.00
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A25742
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Applied Biosystems™
A25742
5 mL
Each for $479.00
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A25743
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Applied Biosystems™
A25743
50 mL
Each for $4,000.00
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A25778
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Applied Biosystems™
A25778
10 x 5 mL
Each for $4,050.00
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A25780
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Applied Biosystems™
A25780
5 x 1 mL
Each for $508.00
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A25918
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Applied Biosystems™
A25918
10 x 1 mL
Each for $970.00
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A25776
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A25776
2 x 5 mL
Each for $886.00
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A25777
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Applied Biosystems™
A25777
5 x 5 mL
Each for $2,100.00
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A25779
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Applied Biosystems™
A25779
2 x 1 mL
Each for $208.00
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Description

Description

Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplify targets for accurate gene expression analysis.

  • A dual hot-start mechanism for excellent specificity
  • Highly reproducible CTs over a broad dynamic range
  • Inclusion of UDG to help prevent carryover contamination
  • Stability of pre-assembled reactions for up to 72 hours
  • Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism:
  • Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding
  • PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures
  • In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification

Tight reproducibility in CTs over a wide dynamic range:
  • PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested
  • In addition, this master mix provides efficient amplification over 6 logs of sample input
  • Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality

UDG included for carryover contamination control:
  • The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives

Stability of pre-assembled reactions:
  • The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data
  • This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1

Broad instrument compatibility:
  • PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2
  • It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
  • For optimal results, recommended primer concentrations are 300 to 800nM

Order Info

Shipping Conditions: Wet Ice

Specifications

Specifications

SYBR
qPCR
Fast or Standard
PowerUP™, SYBR™
Wet Ice
2X
Tube
Dual-Lock Taq DNA Polymerase
7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System
Real Time PCR SYBR Master Mix
Gene Expression
DNA
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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