Invitrogen™ PP2A alpha Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA595235

Catalog No. PIPA595235

Description

Human PP2A-alpha shares 100% amino acid (aa) sequence identity with both mouse and rat PP2A-alpha. Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human Jurkat whole cell, human MCF-7 whole cell, human 293T whole cell, human Hela whole cell, human HepG2 whole cell, rat brain tissue, rat PC-12 whole cell, mouse lung tissue, mouse liver tissue, mouse brain tissue, mouse NIH/3T3 whole cell. IHC: human colorectal adenocarcinoma tissue, human esophageal squamous carcinoma tissue, human thyroid cancer tissue, mouse brain tissue, rat brain tissue. ICC/IF: U2OS cell. Flow: K562 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2A alpha and PP2A beta. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.

Specifications

• Antigen: PP2A alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PPP2CA
• Gene Alias: B55-GAMMA; hypothetical protein; IMYPNO; IMYPNO1; Methyl-PPP2CA; MGC33570; PP2A; PP2A alpha; PP2A C terminal; PP2A L9; PP2A Leucine 9; Pp2a1; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; Ppp2ca; PR52; PR55G; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2 catalytic subunit alpha; protein phosphatase 2, catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2,catalytic subunit alpha isozyme; protein phosphatase 2A alpha subunit; protein phosphatase 2A catalytic subunit alpha isoform; protein phosphatase 2a, catalytic subunit, alpha isoform; protein phosphatase type 2A catalytic subunit; protein phosphatase-2A; protein phosphatase-2A-alpha; R75353; replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; unnamed protein product
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 19052, 24672, 5515
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: P63330, P63331, P67775
• Gene Symbols: PPP2CA
• Immunogen: E.coli-derived human PP2A-alpha recombinant protein (Position: M1-L309).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG