MilliporeSigma™ PP2C Isoform delta, Mouse, Unlabeled, Clone: 2F7.1,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1189

Catalog No. MABS1189MI

Description

Protein phosphatase 1D (EC 3.1.3.16; UniProt O15297; also known as Wild-type p53-induced protein phosphatase 1, PP2C-delta, Protein phosphatase 2C isoform delta, Protein phosphatase magnesium-dependent 1 delta) is encoded by the PPM1D (also known as WIP1) gene (Gene ID 8493) in human. Wild-type p53-induced phosphatase 1 (WIP1) or PP2C-delta is a serine/threonine phosphatase. Unlike PP2A or PP1 enzymes, WIP1 functions as a monomer whose catalytic activity is dependents on Mg2+ or Mn2+ and is insensitive to inhibition by okadaic acid or microcystin. Casein kinase 2 (CK2) andPP2C-delta catalyze the phosphorylation and dephosphorylation, respectively, of LSD1 on Ser131 and Ser137 residues. S131/S137 phosphorylation promots LSD1 interaction with RNF168 and RNF168-dependent 53BP1 ubiquitination and subsequent recruitment to DNA damage sites. Other known PP2C-delta targets include ATM (S367, S1981), -H2AX, CHK1 (S345) and CHK2 (T68).

Specifications

• Antigen: PP2C Isoform delta
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified mouse monoclonal antibody IgG1κ in buffer containing 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% Sodium Azide.
• Gene Accession No.: O15297
• Immunogen: GST-tagged recombinant human PP2C-delta internal fragment.
• Quantity: 100 μL
• Research Discipline: Epigenetics & Nuclear Function
• Gene ID (Entrez): NP_003611
• Target Species: Human
• Form: Purified

• Applications: Western Blot
• Clone: 2F7.1
• Conjugate: Unconjugated
• Gene: PPM1D, WIP1
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 2F7.1 targets an internal epitope conserved among the two human spliced isoforms and mouse/rat PP2C-delta (Uniprot O15297, B1WCA0, Q9QZ67).
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Isotype: IgG1 κ

For Research Use Only. Not for use in diagnostic procedures.