PRDM9, Rabbit anti-Mouse, Human, Polyclonal, Millipore Sigma™

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma ABE194725UG

Catalog No. ABE194725UG

Description

Anti-PRDM9, Cat. No. ABE1947, is a highly specific rabbit polyclonal antibody that targets Histone-lysine N-methyltransferase PRDM9 in human and murine cells and has been tested for use in Western Blotting.

Histone-lysine N-methyltransferase PRDM9 (UniProt: Q9NQV7; EC: 2.1.1.43, PR domain zinc finger protein 9, PR domain-containing protein 9, PRDM9) is encoded by PRDM9 (also known as PFM6) gene (Gene ID: 56979) in human. PRDM9 is a zinc-finger protein that catalyzes histone H3 Lysine 4 trimethylation (H3K4me3), but lacks capacity to mono- and dimethylate Lysine 4 of histone H3. H3 Lysine -4 methylation plays a vital role in the transcriptional activation of genes during early meiotic prophase. PRDM9 contains multiple domains, including a Kruppel-associated box (KRAB) domain (aa 23-86), an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain (aa 244-358), and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. PRDM9 is reported to be highly polymorphic, both within and between mammalian species. It is shown that most of the naturally occurring sequence polymorphisms in PRDM9 change the identity of the amino acids contacting DNA and/or the number and arrangement of individual fingers in the DNA-binding zinc-finger domains. This allows PRDM9 variants to target a large number of DNA sequences, thereby expanding the distribution of recombination sites. (Ref.: Baker, CL et al. (2015). PLoS Genet 11(9): e1005512).

Specifications

• Antigen: PRDM9
• Classification: Polyclonal
• Dilution: Western Blotting Analysis: A representaive lot detected PRDM9 in Wild type, but not in KO mouse testis tissue lysate ( Courtesy  of Guccione Lab @ A*STAR( Institute of Molecular Cell Biology, IMCB) ).
• Gene Alias: Histone-lysine N-methyltransferase PRDM9;EC: 2.1.1.43;PR domain zinc finger protein 9;PR domain-containing protein 9
• Host Species: Rabbit
• Molecular Weight of Antigen: ∽90 kda observed; 103.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Quantity: 25 μg
• Research Discipline: Epigenetics & Nuclear Function
• Test Specificity: This rabbit polyclonal antibody detects Histone-lysine N-methyltransferase PRDM9 in human and mouse cells. It targets an epitope within 191 amino acids from the N-terminal half.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG

• Applications: Western Blot
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: PRDM9;PFM6
• Immunogen: GST-tagged recombinant fragment corresponding to 191 amino acids from the N-terminal half of human Histone-lysine N-methyltransferase PRDM9.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse, Human
• Form: Purified