Invitrogen™ Pre-Pro-Vasopressin Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143640

Catalog No. PIPA5143640

Description

Specific for locally produced levels of the ~ 20 kDa uncleaved pre-pro-vasopressin protein. Immunolabeling of the uncleaved pre-pro-vasopressin protein band is completely blocked by preadsorption of the antibody with the peptide used as antigen.

Vasopressin is a nine amino-acid peptide hormone that plays a key role in water and blood pressure homeostasis (Qureshi S., et al 2014). Vasopressin is the end-product of a highly processed 164 amino acid pre-pro-peptide. Processing of the vasopressin pre-pro-peptide results in three distinct peptides with a 1:1:1 ratio: vasopressin, neurophysin II, and copeptin (Arroyo J.P. et al, 2021). Vasopressin is the biologically active hormone, neurophysin II is a carrier molecule for vasopressin, and copeptin is the c-terminal glycosylated end-product. Vasopressin has been thought to be primarily made in the brain, and the sole source of vasopressin stimulating vasopressin V2 receptors in the kidney until recent studies (Arroyo JP, et al 2022). This antibody specially detects uncleaved pre-pro-vasopressin, accurately identifying locally produced vasopressin versus peripheral uptake of hypothalamic vasopressin or related peptides (Arroyo J.P. et al, 2022). This key research tool aided the Arroyo group in discovering immunoreactive vasopressin outside of the brain by recognizing localized vasopressin in human and mouse kidney, specifically in the distal nephron.

Specifications

• Antigen: Pre-Pro-Vasopressin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: AVP
• Gene Alias: ARVP; VP
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11998, 24221, 551
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.25 mg/mL
• Formulation: 10mM HEPES with 100μg/mL BSA, 50% glycerol, 150mM NaCl and no preservative; pH 7.5
• Gene Accession No.: P01185, P01186, P35455
• Gene Symbols: AVP
• Immunogen: Synthetic peptide corresponding to key amino acid residues surrounding the cleavage sites for the human vasopressin precursor peptide, conjugated to keyhole limpet hemocyanin (KLH).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG