Invitrogen™ PREB Recombinant Rabbit Monoclonal Antibody (JE64-56)

Catalog No.	Quantity
FEMA550674	100 μL

Catalog No. FEMA550674 Supplier Invitrogen™ Supplier No. MA550674

Description

Rabbit Recombinant Monoclonal Antibody

Positive control: HepG2 cell lysates, human lymph nodes tissue, human colon tissue. Predicted band size: 45 kDa Subcellular Location: Endoplasmic reticulum membrane, Nucleus.

Prolactin (PRL) expression in the pituitary is limited to specific cells. Pit-1 is a pituitary specific transcription factor that plays an important role in PRL expression, both in mature organism and during development. The PRL promoter contains numerous Pit-1 binding sites and these sites have been implicated in both basal level and kinase-mediated gene expression. The most proximal of these binding sites, termed 1P, has been shown to direct a response to numerous signals, such as cAMP and various G-proteins. A novel protein, termed PREB (prolactin regulatory element binding protein), has been recently identified that regulates PRL gene expression through the 1P site, though it contains no discernable DNA-binding motif. Recent studies suggest that PREB is encoded by a single-copy gene in both mice and humans and exhibits nuclear accumulation in pituitary cells. Evidence suggests that PREB is a novel transcription factor that assists in PRL expression whether alone, or in concert with Pit-1.

Specifications

• Antigen: PREB
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Recombinant Monoclonal
• Clone: JE64-56
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: TBS with 0.05% BSA, 40% glycerol and 0.05% sodium azide; pH 7.4
• Gene: Preb
• Gene Accession No.: Q9HCU5
• Gene Alias: C85705; mammalian guanine nucleotide exchange factor mSec12; PREB; prolactin regulatory binding-element protein; prolactin regulatory element binding; prolactin regulatory element binding protein; prolactin regulatory element-binding protein; Sec12
• Gene Symbols: Preb
• Host Species: Rabbit
• Immunogen: Recombinant protein within human PREB aa 81-230/417.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 10113
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG