Invitrogen™ PRKAR1B Polyclonal Antibody

Catalog No.	Quantity
PIPA547592	100 μg

Catalog No. PIPA547592 Supplier Invitrogen™ Supplier No. PA547592

Description

Sheep Polyclonal Antibody

In Western blots, less than 1% cross-reactivity with recombinant human (rh) PKA RI alpha or rhPKA RII beta is observed; cross-reactivity with PKA RII alpha is unknown. Reconstitute at 0.2 mg/mL in sterile PBS.

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified that each represent specific gene products. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcrip- tion factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Specifications

• Antigen: PRKAR1B
• Applications: Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 5% trehalose and No Preservative
• Gene: PRKAR1B
• Gene Accession No.: P12849, P31321, P81377
• Gene Alias: AI385716; cAMP dependent protein kinase, subunit type 1 beta; cAMP dependent regulatory type I beta subunit transcript 2; cAMP dependent regulatory type I beta subunit transcript 3; cAMP dependent regulatory type I beta subunit transcript 4; cAMP-dependent protein kinase type I-beta regulatory subunit; PRKAR1; PRKAR1B; protein kinase cAMP-dependent type I regulatory subunit beta; protein kinase, cAMP dependent regulatory, type I beta; protein kinase, cAMP dependent regulatory, type I, beta; protein kinase, cAMP-dependent, regulatory subunit type I beta; protein kinase, cAMP-dependent, regulatory, type I, beta; RGD1563094; RIbeta
• Gene Symbols: PRKAR1B
• Host Species: Sheep
• Immunogen: E. coli-derived recombinant human PKA RI beta Met1-Val381.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 19085, 25521, 5575
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov