Invitrogen™ pro-Caspase 3 Recombinant Rabbit Monoclonal Antibody (SZ02-08)

Catalog No.	Quantity
PIMA532027	100 μL

Catalog No. PIMA532027 Supplier Invitrogen™ Supplier No. MA532027

Description

Rabbit Recombinant Monoclonal Antibody

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa.

Specifications

• Antigen: pro-Caspase 3
• Applications: Immunohistochemistry (Paraffin), Western Blot, Western Blot, Western Blot
• Classification: Recombinant Monoclonal
• Clone: SZ02-08
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: TBS with 40% Glycerol, 0.05% BSA and 0.05% sodium azide; pH 7.4
• Gene: CASP3
• Gene Accession No.: P42574, P70677
• Gene Alias: A830040C14Rik; AC-3; Apopain; Casp3; CASP-3; caspase 3; caspase 3, apoptosis related cysteine protease; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; caspase-3; Caspase-3 subunit p12; Caspase-3 subunit p17; CC3; Cpp32; CPP-32; CPP32B; cysteine protease CPP32; LICE; mldy; PARP cleavage protease; procaspase3; protein Yama; SCA-1; SREBP cleavage activity 1; Yama
• Gene Symbols: CASP3
• Host Species: Rabbit
• Immunogen: Synthetic peptide within Human pro Caspase-3 aa 18-67.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12367, 836
• Target Species: Human, Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG