R&D Systems™ ProDots Human IGF-I Recombinant Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The ProDots Recombinant Human IGF-I/IGF-1 Protein is derived from E. coli. The ProDots Recombinant Human IGF-I/IGF-1 Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: P05019
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 3479
• Molecular Weight (g/mol): Observed M.W.: 7kDa, reducing conditions, Theoretical M.W.: 7.6kDa
• Name: IGF-I/IGF-1
• Quantity: 50 μg
• Source: E. coli-derived human IGF-I/IGF-1 protein Gly49-Ala118
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt at room temperature as supplied. 12 months from date of receipt at 2-8° C as supplied. 1 month at 2-8° C under sterile conditions after reconstitution. 3 months at -20 to -80° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: IBP1, IGF1, IGF-1, IGF1A, IGFI, IGF-I, IGF-IA, IGF-IB, insulin-like growth factor 1, insulin-like growth factor 1 (somatomedin C), insulin-like growth factor I, insulin-like growth factor IA, insulin-like growth factor IB, Mechano growth factor, MGF, Somatomedin A, Somatomedin C, Somatomedin-C
• Common Name: IGF-I
• Gene Symbol: IGF1
• Biological Activity: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 0.3-1.5ng/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: For a stock solution, reconstitute at 200 μg/mL in sterile PBS, or simply roll ProDot™ directly into cell culture medium for immediate use.
• Expression System: E. coli-derived Gly49 - Ala118
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain