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Prol1 Antibody, ProSci
SDP

Catalog No. 16822106
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16-822-106 0.1 mg
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Catalog No. 16-822-106 Supplier ProSci LLC Supplier No. 423110100
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Description

Goat Polyclonal Antibody

Prol1 Polyclonal Antibody specifically detects Prol1 in Mouse samples. It is validated for ELISA, Immunohistochemistry

Specifications

Antigen Prol1
Applications ELISA, Immunohistochemistry
Classification Polyclonal
Concentration 500 ug/mL
Conjugate Unconjugated
Dilution Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 45kDa band in Mouse Eye lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.6kDa according to NP_032670.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:Positive staining in the submandibular salivary gland of the mouse, while cells remain negative in the sublingual salivary gland. Data provided by Everest Grant winner Melinda Larsen State University of New York, Albany, NY. A publication from this author includes the use of this antibody: Nelson at al, Biol Open. 2013 Apr 18;2(5):439-47, PMID: 23789091.
Formulation Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Gene Accession No. NP_032670.2
Gene Alias Prol1, proline rich, lacrimal 1, Muc10, mucin 10, mucin 10, submandibular gland salivary mucin
Host Species Goat
Immunogen The immunogen for this antibody is: C-QFPVRKYLEDPRY
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Quantity 0.1 mg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 17830(mouse);
Target Species Mouse
Content And Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Product Type Antibody
Form Liquid
Isotype IgG
Research Category Other
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