Invitrogen™ Prolactin Receptor Monoclonal Antibody (U5)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA1610

Catalog No. MA1610

Description

MA1-610 detects prolactin (PRL) receptor from rat, human, rabbit, horse, and pig tissues. MA1-610 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, and FACS procedures. By Western blot, this antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. Immunohistochemical staining of PRL receptor in rat Islets of Langerhans with MA1-610 results in a staining pattern consistent with cytoplasmic vesicle staining. MA1-610, after biotinylation, has been used to detect human lymphocyte PRL receptor by FACS. MA1-610 does not inhibit the interaction of prolactin with the receptor. The MA1-610 immunogen is purified PRL receptor from rat liver. This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain. MA1-610 does not inhibit the interaction of prolactin with the receptor. Reconstitute with 100 μL PBS.

Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Specifications

• Antigen: Prolactin Receptor
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: P05710, P14787, P16471, Q08501, Q6JTA8
• Gene Symbols: PRLR
• Immunogen: Purified rat liver PRL receptor.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Rabbit, Pig, Horse
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: U5
• Conjugate: Unconjugated
• Gene: PRLR
• Gene Alias: AI987712; cytokine receptor; HPRL; hPRL receptor; hPRLrI; Lactogen receptor; MFAB; pPRLR-SF; Pr-1; Pr-3; Prl R; PRLR; PRL-R; Prlr-rs1; Prolactin receptor; prolactin receptor 2 precursor; prolactin receptor related sequence 1; prolactin receptor short isoform; RATPRLR; secreted prolactin binding protein
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 100009046, 100053793, 19116, 24684, 414916, 5618
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Lyophilized