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Promega Alkaline Phosphatase, Calf Intestinal

Catalog No. PRM2825 Shop All Promega Corporation Products
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Concentration:
1 U/μL
20 U/μL
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Catalog No. Concentration
PR-M2825 20 U/μL
PR-M1821 1 U/μL
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Catalog No. PR-M2825 Supplier Promega Supplier No. M2825
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Description

Alkaline Phosphatase catalyzes the hydrolysis of 5'-phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates.

  • Catalyzes the Hydrolysis of 5′ Phosphate Groups from DNA, RNA, and r- and dNTPs
  • Available at high concentration; Cat.# M2825 contains 1,000 units of CIAP at 20u/μL
  • Qualified for blue/white cloning; our assay provides a higher level of quality control for enzymes used in cloning applications

Specifications

Concentration 20 U/μL
Color Colorless
Components 10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
pH 8
For Use With (Application) Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
Storage Buffer 10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
Content And Storage -30°C to -10°C
Source Calf intestinal mucosa
Quantity 1000 U
Product Type Alkaline Phosphatase
Form Liquid
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