Promega Alkaline Phosphatase, Calf Intestinal
Alkaline Phosphatase catalyzes the hydrolysis of 5 ft.-phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates.
Manufacturer: Promega M2825
- Catalyzes the Hydrolysis of 5 ft. Phosphate Groups from DNA, RNA, and r- and dNTPs
- Available at high concentration; Cat.# M2825 contains 1,000 units of CIAP at 20u/µL
- Qualified for blue/white cloning; our assay provides a higher level of quality control for enzymes used in cloning applications
|10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine|
|Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase|
|−30 to −10°C|
|Calf intestinal mucosa|
|10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol|
Purchase up to $1,000 in Promega reagents and get a matching amount of products at no additional cost.