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Promega Alkaline Phosphatase, Calf Intestinal

Alkaline Phosphatase catalyzes the hydrolysis of 5 ft.-phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates.

Manufacturer:  Promega M2825

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Catalog No. PR-M2825

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  • Catalyzes the Hydrolysis of 5 ft. Phosphate Groups from DNA, RNA, and r- and dNTPs
  • Available at high concentration; Cat.# M2825 contains 1,000 units of CIAP at 20u/µL
  • Qualified for blue/white cloning; our assay provides a higher level of quality control for enzymes used in cloning applications


Alkaline Phosphatase
10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
−30 to −10°C
Calf intestinal mucosa
10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol


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