Promega AMV Reverse Transcriptase

AMV RT catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids.

Supplier:  Promega M5101

Catalog No. PR-M5101

Includes: 5X Reaction Buffer (250mM Tris-HCl [pH 8.3 at 25°C], 250mM KCl, 50mM MgCl₂, 2.5mM spermidine, 50mM DTT)

Description

• Efficient Reverse Transcription of Templates with High Secondary Structure
• DNA polymerization using template DNA, RNA or RNA:DNA hybrids
• Requires a primer as well as magnesium or manganese
• Stable at higher reaction temperatures (37-58°C)
• Supplied at a concentration of 10u/µL (Cat.# M5101, M5108) or 20-25u/µL (Cat.# M9004)

Specifications

• Quantity: 300U
• Concentration: 10U/μL
• Format: Liquid
• For Use With (Application): First and second strand synthesis of cDNA, primer extensions and RNA sequencing
• Product Name: AMV Reverse Transcriptase

• Storage Requirements: −30 to −10°C
• Source: Avian Myeloblastosis Virus
• Storage Buffer: Potassium Phosphate (pH 7.2 at °C) 200mM, 0.2% Triton™ X-100, DTT 2mM and Glycerol 50%
• Includes: 1 x 500μL 5X reaction buffer