Promega Arg-C Sequencing Grade Modifying Enzyme

Catalog No.	Quantity
PRV1881	10 μg

Catalog No. PRV1881 Supplier Promega Supplier No. V1881

Description

Arg-C (clostripain) is an endopeptidase that cleaves at the C-terminus of arginine residues, including the sites next to proline. Cleavage also will occur at lysine residues. Arg-C activity is optimal in the pH range of 7.6-7.9.

• Sequencing-Grade Protease: for use Alone or with other Proteases for Mass Spectrometry Analysis
• Cleaves at the C-terminus of arginine residues, including sites next to proline
• Also cleaves at lysine residues
• Activity is optimal in the pH range of 7.6-7.9

Specifications

• Color: Colorless
• pH: 7.6 to 7.9
• For Use With (Application): Peptide mapping, Protein identification, characterization of post-translational modifications
• Storage Buffer: 50mM Tris HCl (pH 7.6 to 7.9), 5mM CaCl2, 2mM EDTA
• Content And Storage: 2°C to 10°C
• Source: Clostridium histolyticum
• Quantity: 10 μg
• Product Type: Arg-C (clostripain)
• Molecular Weight (g/mol): 45 and 12
• Inhibitors: Oxidizing agents and Sulfhydryl reactants, also Co2+, Cu2+, Cd2+ and heavy Metal Ions, Citrate, Borate and Tris anions partially inhibit activity, TLCK (Tosyllysine Chloromethyl Ketone) reacts with active site (1,5,6)
• Form: Lyophilized Powder