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Promega GeneClip™ U1 Hairpin Cloning Systems

Designed for fast and easy cloning of human hairpin target sequences to allow expression of siRNA target sequences in human cells

Specifications

Format Liquid
For Use With (Application) Fast and easy cloning of Human hairpin target sequences to allow expression of siRNA target sequences in Human cells
pH 7.4
Quantity 20 Reactions
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Includes: 2X Rapid Ligation Buffer, 200µL; Nuclease-free Water, 1.25mL; Oligo Annealing Buffer, 1mL; pGeneClip Vector (varies with system), 1.2µg; T4 DNA Ligase, 100U

Products 1
Catalog Number Mfr. No. Includes Price Quantity & Availability  
Catalog Number Mfr. No. Includes Price Quantity & Availability  
PRC8790
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Promega
C8790
200μL 2X rapid Ligation Buffer, 1.25mL Nuclease-free Water, 1mL Oligo annealing Buffer, 1.2μg pGeneClip hMGFP vector, 100U T4 DNA Ligase This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
Description

Description

Use pGeneClip™ vectors containing the human U1 promotor, which allows transcription of the hairpin target sequences and generation of hairpin siRNA in vivo.

  • Utilize predigested vectors to eliminate time-consuming vector preparation
  • Allow easier identification of desired clones using a Pst I digestion

  • Available with a choice of vectors
    • The hMGFP System enables easy determination of transfection efficiency
    • The other systems offer antibiotic marker options for selecting stably transfected eukaryotic cells so that experimental results do not depend on transfection efficiency

For nonprofit and noncommercial research use only. With respect to commercial research use or use by for-profit organizations, or any diagnostic or therapeutic uses, please contact Promega Corporation for supply and licensing information.
Licensed under U.S. Patent 6,573,099 and its foreign equivalents from Benitec Australia Ltd and CSIRO.
This product is covered under license from Carnegie Institution of Washington under U.S. Pat. Nos. 6,506,559, 7,538,095, 7,560,438; Australian Patent 743798 and other patents pending. Commercial use of this product may require a separate license from Carnegie.
For research use only. With respect to any and all other uses, please contact Promega Corporation for supply and licensing information.
Certain applications of this product may require licenses from others.

Specifications

Specifications

Liquid
Fast and easy cloning of Human hairpin target sequences to allow expression of siRNA target sequences in Human cells
7.4
20 Reactions
SDS
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